FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14883329
Sequences flagged as poor quality0
Sequence length31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAATACCTGCCACCCCACTCTTAATCAG178240.11975815356900328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCCG90750.015.27709625
AGCCGGT17350.014.91261410
GTATCAA120400.014.8354251
TATGCGA12800.013.5748775
TCGTGTA10350.013.40676323
ACTCTTA39600.013.09989820
GATTAAT41800.012.7177691
CACTCTT39350.012.70641319
ATATGCG13450.012.6398914
ATGTCCG23900.012.34178715
CGGTGTA21850.012.18406313
ATACGAC8550.012.1344419
TCTAAAG40500.012.0364882
CTAATAC54950.012.0104933
CCCTAAT45650.012.0013921
ACGGTAG7500.011.99993719
CCTAATA50300.011.9030352
GCGAATC14250.011.8413678
TCGGGCG7150.011.71208411
TCCGCGG10950.011.64506625
Produced by FastQC (version 0.11.5)