FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14075040
Sequences flagged as poor quality0
Sequence length31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAATACCTGCCACCCCACTCTTAATCAG169370.12033358342143255No Hit
GCCATATATTGCATGGGGGTACCCCAATCTG152630.10844018915754414No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAG146870.10434783844308791No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGGT17250.015.72276510
GCTTCCG78000.014.91899625
ATGTCCG21300.014.19988415
GTATCAA106500.014.1148781
CGGTGTA19550.013.93606713
GTCGTAT2600.013.468121
TCGTGTA9550.013.21883823
AACGGTA6150.013.00736618
CCCTAAT40450.012.5215691
ACTCTTA38300.012.33609120
GATTAAT43950.012.2073311
CCTAATA45450.012.1339522
CTAATAC50150.012.1189353
TATGCGA12500.012.1050555
ACGGTAG6550.012.02219519
TCTAAAG40400.011.9791822
CACTCTT38750.011.80575819
TGGACTG36250.011.7980295
ATATGCG12800.011.6268994
TCTATAC28000.011.6121983
Produced by FastQC (version 0.11.5)