FastQCFastQC Report
Wed 8 May 2024
EGAF00004824122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14772635
Total Bases457.9 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGACGTACTCTGCGTTGATACCACTGC277650.18794886626522622Clontech SMART CDS Primer II A (95% over 24bp)
GACGTACTCTGCGTTGATACCACTGCTTCCG261870.17726695339050888Clontech SMART CDS Primer II A (96% over 26bp)
CCCTAATACCTGCCACCCCACTCTTAATCAG210400.1424255049962312No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCG166370.11262039575201038Clontech SMART CDS Primer II A (96% over 26bp)
CTAGACGTACTCTGCGTTGATACCACTGCTT159740.10813236771909683Clontech SMART CDS Primer II A (96% over 26bp)
GACTTAAACAGCTTAAAGTTTAGTTTAAAAG157470.1065957427364854No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATT153510.10391511060822935No Hit
AACGTACTCTGCGTTGATACCACTGCTTCCG150210.10168125050134928Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)