FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14772635
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGACGTACTCTGCGTTGATACCACTGC277650.18794886626522622No Hit
GACGTACTCTGCGTTGATACCACTGCTTCCG261870.17726695339050888No Hit
CCCTAATACCTGCCACCCCACTCTTAATCAG210400.1424255049962312No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCG166370.11262039575201038No Hit
CTAGACGTACTCTGCGTTGATACCACTGCTT159740.10813236771909683No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAG157470.1065957427364854No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATT153510.10391511060822935No Hit
AACGTACTCTGCGTTGATACCACTGCTTCCG150210.10168125050134928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCCG115600.017.10874225
AGCCGGT14900.015.09956610
ACGGTAG6400.014.25802519
AACGGTA6750.014.07423518
TGCTTCC146700.013.71182824
GTATCAA127250.013.6338211
ACGACTT8000.013.59445821
ATGTCCG20200.013.30307715
TAGGTCG2750.012.7270765
CGGTGTA18200.012.43011513
GATTAAT54100.012.4158341
ACTCTTA43900.012.35827520
CGGTAGT6950.012.23085420
CCGGTGT18300.012.15720912
CCCTAAT48600.012.1479951
CTAATAC57500.011.9120913
CACTCTT44950.011.79105419
GAGTGGT35100.011.75095911
CCTAATA55550.011.6776162
TTAATTG38300.011.617873
Produced by FastQC (version 0.11.5)