FastQCFastQC Report
Wed 8 May 2024
EGAF00004824124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14724497
Total Bases456.4 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAGACGTACTCTGCGTTGATACCACTGC279020.18949373958241153Clontech SMART CDS Primer II A (95% over 24bp)
GACGTACTCTGCGTTGATACCACTGCTTCCG269450.1829943664629087Clontech SMART CDS Primer II A (96% over 26bp)
CCCTAATACCTGCCACCCCACTCTTAATCAG209180.1420625777573251No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCG168100.11416349230809039Clontech SMART CDS Primer II A (96% over 26bp)
CTAGACGTACTCTGCGTTGATACCACTGCTT163070.11074741636335694Clontech SMART CDS Primer II A (96% over 26bp)
GACTTAAACAGCTTAAAGTTTAGTTTAAAAG158490.10763695357471294No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATT155610.1056810293757403No Hit
AACGTACTCTGCGTTGATACCACTGCTTCCG153120.10398996991204522Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)