FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15378345
Sequences flagged as poor quality0
Sequence length31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAATACCTGCCACCCCACTCTTAATCAG176480.11475877280682674No Hit
GCCATATATTGCATGGGGGTACCCCAATCTG158090.10280039887257048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCCG83550.015.16977825
GTATCAA110150.014.3967391
TCGTGTA12350.013.86505423
AGCCGGT17700.013.55805410
GACCGTC9350.013.5008847
GATTAAT51000.012.9967581
ACGACTT10400.012.97959721
AACGGTA6300.012.8956618
TATGCGA15100.012.8367315
TACGACT8700.012.786201520
ACGGTAG6350.012.59728819
ATATGCG15450.012.5467494
ATACGAC8700.012.49887119
CGTTGTT12750.012.44993724
CTCGTGT14200.012.23481122
ACTCTTA39950.012.13907920
CCCTAAT45300.012.03698351
AACGTTG14750.012.03281222
GCGAATC16100.011.9551318
CCTAATA49750.011.8647862
Produced by FastQC (version 0.11.5)