FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15120700
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA406720.26898225611248155No Hit
AAAAAAAAAAAAAAAAAAAAAATAA197590.13067516715495975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTTT1045900.019.33796919
CGTTTTT1006400.019.31809219
GCTTTTT1064550.019.31276919
GATTTTT1210050.019.29848719
TGTTTTT1386950.019.29507419
CATTTTT778150.019.28605519
AATTTTT870550.019.28192919
TCTTTTT716250.019.27960219
GGTTTTT2103200.019.2752919
CCTTTTT505150.019.25424219
TAATTTT232050.019.22813818
ACTTTTT623050.019.22697419
GGATTTT459450.019.17197418
AGTTTTT1181300.019.16796719
CGGTTTT396900.019.1639918
GCGTTTT390750.019.1405118
CCGTTTT184250.019.12841218
GGCTTTT417250.019.12616218
TTATTTT292650.019.11617518
AGGTTTT388800.019.10986918
Produced by FastQC (version 0.11.5)