FastQCFastQC Report
Wed 8 May 2024
EGAF00004824132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15559361
Total Bases482.3 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGTCTCTAAAAATCTCTAAAAATC525650.33783521058480487No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCG182690.11741484756346998Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)