FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15559361
Sequences flagged as poor quality0
Sequence length31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGTCTCTAAAAATCTCTAAAAATC525650.33783521058480487No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCG182690.11741484756346998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCCG100750.015.66120925
GTATCAA160950.014.7267431
TATGCGA14400.013.6260325
AGCCGGT20900.013.51636910
AACGGTA10500.012.858644518
ATATGCG15300.012.578844
CGGTGTA22200.012.55704713
GGTATCA66600.012.5511121
TCGTGTA10900.012.50282823
GACCGTC11400.012.4982857
ATACGAC7100.012.32566219
GCGAATC15800.012.2615288
ACGGTAG11250.012.22392519
ACGACTT9250.012.16448321
TGCTTCC130900.012.11129724
TCCGCGG12750.012.06162825
TATACTG35250.011.8773065
TCTATAC32000.011.71567253
GATTAAT38300.011.6466161
ACGGTCG2800.011.60984324
Produced by FastQC (version 0.11.5)