FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18629457
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA295960.15886668086997918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTTT1298050.019.34055919
AATTTTT1088050.019.29213319
TCTTTTT907450.019.2749219
TGTTTTT1723800.019.27216119
GCTTTTT1307400.019.25788719
GATTTTT1500200.019.25145719
CGTTTTT1275750.019.24863819
CCTTTTT636000.019.2335119
ACTTTTT779450.019.21140919
CATTTTT968200.019.19968219
GCGTTTT494000.019.16222618
GGTTTTT2599000.019.15935319
GGATTTT561700.019.13667118
AGTTTTT1458300.019.1351819
GTATTTT429000.019.1071718
ATATTTT289500.019.0878418
CGGTTTT486900.019.08427618
TAATTTT296750.019.05548118
GGCTTTT494900.019.04792418
AAATTTT243600.019.03216718
Produced by FastQC (version 0.11.5)