FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18629457
Sequences flagged as poor quality0
Sequence length31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGTCTCTAAAAATCTCTAAAAATC256500.13768517246637946No Hit
CCCCTATACCTTCTGCATAATGAATTAACTA216700.11632115740142077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCCG107450.015.63795425
GTATCAA142850.014.39871
TCGTGTA14600.013.95788223
ACGACTT10800.013.65935321
ACGGTAG10250.013.53829819
AGCCGGT22050.013.49213810
ATACGAC8700.013.07636519
AACGGTA11000.012.84222118
TACGACT9600.011.98077820
CGGTGTA24650.011.9681613
CGGTAGT11500.011.8494220
GATTAAT49050.011.8209571
TATGCGA16500.011.8163775
ATATGCG17000.011.6887634
TGCTCGT18300.011.61358320
CGACTTT9700.011.59984122
TGCTTCC145650.011.55374524
ATGTCCG20800.011.5386515
GACCGTC11050.011.5377837
GTATTGG39350.011.4322431
Produced by FastQC (version 0.11.5)