FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19242368
Sequences flagged as poor quality0
Sequence length31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGTCTCTAAAAATCTCTAAAAATC260790.13552905754634773No Hit
CCCCTATACCTTCTGCATAATGAATTAACTA223970.11639419846871236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCCG111100.015.64379825
AGCCGGT22650.014.56939810
GTATCAA144800.014.4787721
TCGTGTA16500.013.41304423
TATGCGA15050.013.369295
ACGGTAG10150.013.1793419
AACGGTA10450.012.92025118
CGGTGTA25700.012.84121413
ACGACTT11650.012.66362321
ATACGAC9300.012.63632619
AACGTTG18300.012.50297122
ATGTCCG19800.012.18511515
CAACGTT19100.012.04443421
GATTAAT49750.012.0295881
ATATGCG16600.011.9692614
TCCGCGG14000.011.87859325
TGTACGC8700.011.78514424
TGCTTCC150750.011.66187424
CGTTGTT18850.011.541923524
TATACTG42250.011.5360435
Produced by FastQC (version 0.11.5)