FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18965259
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA228620.12054673231723331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTTT1333300.019.32674219
AATTTTT1119400.019.32193419
CGTTTTT1303900.019.30932619
GATTTTT1551350.019.28928819
CCTTTTT641050.019.28871519
TGTTTTT1757100.019.28006419
AGTTTTT1481900.019.26175119
GCTTTTT1348700.019.25478419
TCTTTTT919550.019.253919
CATTTTT1001000.019.22444519
ACTTTTT790950.019.19708819
GGATTTT586850.019.18871918
GGTTTTT2652250.019.18367419
GCGTTTT509050.019.18167118
TAATTTT303250.019.12956418
TTATTTT380250.019.10861218
GTATTTT437450.019.10713218
CGGTTTT503100.019.10560218
TACTTTT208700.019.07564518
TAGTTTT374900.019.06133818
Produced by FastQC (version 0.11.5)