FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18965259
Sequences flagged as poor quality0
Sequence length31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTA218240.11507356688353162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA13600.014.4327123
AGCCGGT22900.014.13752910
GTATCAA139850.014.1001321
GCTTCCG103350.014.02053725
TATGCGA15000.013.9146975
AACGGTA9550.013.35224118
ATATGCG15850.012.7732064
ACGGTAG10000.012.75152519
CGGTGTA26700.012.35998513
CGATCAT9050.012.29500123
TCTATAC39350.012.16360953
ACGACTT12050.012.13866921
GACCGTC11350.012.0036247
GCGAATC17200.011.9178928
ATGTCCG23400.011.91278115
AACGTTG14700.011.821671522
TATACTG41850.011.736695
CGTGTAG17050.011.7323524
TCCGCGG14500.011.72634425
TCGTAAG5300.011.5528652
Produced by FastQC (version 0.11.5)