FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32527172
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA437720.1345705676472581No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCC964150.018.909085
TCGCATC958550.018.8935264
TACTCCA364100.018.8276183
CTCCTAC298700.018.819724
CTACTCC365500.018.814232
CACCTTC508250.018.8139423
CCTATAG446250.018.8012182
TCCTTAC404800.018.8002934
CCTCTGA402850.018.7968524
AACTGCA601250.018.7909953
GCCTATA447000.018.7891431
TCCTCTG403850.018.7880213
TTGTCGC966750.018.7846071
CCTTACG407700.018.7832165
TCAACCG302200.018.7777921
TATAGCA444300.018.775184
GGTCCAA489050.018.7668133
GCATCCA973450.018.755916
AAGGTCC490550.018.7503681
CTATCCG681450.018.7490886
Produced by FastQC (version 0.11.5)