FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32527172
Sequences flagged as poor quality0
Sequence length31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA300500.014.6918251
GCTTCCG136400.014.478682525
AGCCGGT28100.013.07671410
GGTATCA127400.012.8111071
GACCGTC20350.012.6514137
TGGACTG98250.012.0790965
TATGCGA29750.012.0641215
TCGTGTA25950.012.04128823
ATATGCG29850.011.9827144
GATTAAT103300.011.9740621
AACGTTG31450.011.64445922
ACGGTAG11500.011.41205219
GGACTGA86750.011.4102896
ATGTCCG44050.011.29223915
GCGAATC31900.011.2835588
CCCTAAT73800.011.1001921
TCTAAAG78600.011.0582672
GTATTGG79500.011.0279911
ATACGAC15550.011.01190519
ACCGTCT25350.010.9450878
Produced by FastQC (version 0.11.5)