FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12706572
Sequences flagged as poor quality0
Sequence length31
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTA151140.11894632163576456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCCG60750.014.6938425
AACGGTA6900.014.13176518
ACGGTAG6900.013.76979219
AGCCGGT15400.013.31142110
GTATCAA87050.012.86282351
TATGCGA10850.012.7858515
CCTACGG10400.012.7367712
CGTTGTT10650.012.67819924
AACGTTG11150.012.67015322
TCGTGTA9100.012.63959823
TGGACTG35900.012.602325
GCCTACG10650.012.4380781
ATGTCCG17300.012.42818315
GACCGTC10100.012.3752597
TCTAAAG30250.012.2692462
ATATGCG11350.012.222264
TATACTG30400.012.1689885
TCTATAC28000.012.0957353
GGACTGA31850.012.0471926
TAGGTCG3450.011.9544945
Produced by FastQC (version 0.11.5)