FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13135184
Sequences flagged as poor quality0
Sequence length31
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTA155120.11809503391806311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTCCG61800.014.66923325
AACGGTA6200.014.11518218
TCGTGTA9950.013.94904723
ACGGTAG6550.013.55216719
GACCGTC10300.013.4682257
TATGCGA10950.013.1245215
TGGACTG34150.013.1006285
AACGTTG10750.012.91029122
AGCCGGT15300.012.8266210
GTATCAA85700.012.7414241
ATGTCCG16650.012.5385115
GGACTGA30150.012.5176956
ATATGCG11700.012.4961614
TCTATAC28750.012.2566393
GATTAAT29700.012.1570641
ACCGTCT12250.012.1408858
TATACTG30950.012.0728645
TCTAAAG31600.012.0598362
TGGACCG15300.011.8433995
GTCTTAC19900.011.740211
Produced by FastQC (version 0.11.5)