FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23733860
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACC491400.018.8612163
CGTTAGA492100.018.8467141
CCTACAA232300.018.842771
AGACCAG439000.018.8305721
CACTGAC397650.018.8136023
CAGCACA439250.018.8085785
ACTTACC484650.018.8049876
CGCATCC385950.018.8041485
TACTCCA290750.018.7997533
AACTACC208950.018.7989986
CCAGCAC439850.018.7949894
CTTCGTC375950.018.7875422
CGGAATA327550.018.778531
CCACTGA398550.018.7758852
CACCTTC304350.018.7713453
TACACCT304600.018.7673151
CTACTCC291950.018.7647882
TCGCATC386700.018.7615744
AGGAACA393800.018.761092
GACGAGA543500.018.7598485
Produced by FastQC (version 0.11.5)