FastQCFastQC Report
Fri 10 May 2024
EGAF00004824152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10557236
Total Bases327.2 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTACTCTGCGTTGATACCACTGCTTCCG137770.13049817205942918Clontech SMART CDS Primer II A (96% over 26bp)
AACGTACTCTGCGTTGATACCACTGCTTCCG131080.1241612861548231Clontech SMART CDS Primer II A (96% over 26bp)
TACGTACTCTGCGTTGATACCACTGCTTCCG130420.12353612252297855Clontech SMART CDS Primer II A (96% over 26bp)
GCCATATATTGCATGGGGGTACCCCAATCTG109940.10413710558331746No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)