FastQCFastQC Report
Wed 8 May 2024
EGAF00004824160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17940881
Total Bases556.1 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTACTCTGCGTTGATACCACTGCTTCCG274170.15281858232045573Clontech SMART CDS Primer II A (96% over 26bp)
GTGAATGGGTCTCTAAAAATCTCTAAAAATC267360.14902278210306397No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCG243840.13591305800423067Clontech SMART CDS Primer II A (96% over 26bp)
GACGTACTCTGCGTTGATACCACTGCTTCCG202740.11300448400499395Clontech SMART CDS Primer II A (96% over 26bp)
AACGTACTCTGCGTTGATACCACTGCTTCCG198870.11084739929995635Clontech SMART CDS Primer II A (96% over 26bp)
CCCCTATACCTTCTGCATAATGAATTAACTA194270.10828342264797364No Hit
GCCATATATTGCATGGGGGTACCCCAATCTG184090.10260923083989018No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)