FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18511046
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA567440.3065412943169176No Hit
ACTGTCTCTTATACACATCTCCGAG283700.15325984279872676No Hit
GCTGTCTCTTATACACATCTCCGAG253310.13684261818591992No Hit
CCTGTCTCTTATACACATCTCCGAG213480.11532573577959883No Hit
TCTGTCTCTTATACACATCTCCGAG204980.11073388289348965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACC288000.018.9185521
CCAACGA288450.018.8408666
GCCTATA258000.018.8363111
GGATACC378700.018.8233556
TCCACAC238100.018.8221051
AGACCAG334400.018.8120141
TCGACCA289500.018.80892
CCTCTGA250550.018.8053444
AGTCCTC251500.018.8003751
AAGGTCC248950.018.793951
TTCCAGA260400.018.7907581
CCTATAG258450.018.7893162
TATAGCA256100.018.787364
CTACACG271300.018.7697351
CTACTCC150750.018.7693392
CTTCGTC215550.018.764512
CCAGCAC335150.018.7644024
AGGAACA305650.018.763182
TACTCCA150100.018.7619973
CAGCACA334550.018.759975
Produced by FastQC (version 0.11.5)