FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004824162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18511046
Sequences flagged as poor quality0
Sequence length31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTACTCTGCGTTGATACCACTGCTTCCG276210.14921361007908465Clontech SMART CDS Primer II A (96% over 26bp)
GTGAATGGGTCTCTAAAAATCTCTAAAAATC273650.1478306520333859No Hit
CACGTACTCTGCGTTGATACCACTGCTTCCG249220.13463312662072147Clontech SMART CDS Primer II A (96% over 26bp)
GACGTACTCTGCGTTGATACCACTGCTTCCG206490.11154961205325728Clontech SMART CDS Primer II A (96% over 26bp)
AACGTACTCTGCGTTGATACCACTGCTTCCG201380.10878909814172576Clontech SMART CDS Primer II A (96% over 26bp)
CCCCTATACCTTCTGCATAATGAATTAACTA200580.10835692375244488No Hit
GCCATATATTGCATGGGGGTACCCCAATCTG186950.10099375259507216No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)