FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24347040
Sequences flagged as poor quality0
Sequence length89
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10125464.158805341429595No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7370173.027131840256557No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7352923.02004679008208No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6926962.8450932844403263No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6885152.8279207657275793No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6511312.6743743798014052No Hit
TGATGGCCTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6464682.655222154315268No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6242642.564024209924492No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5617122.3071059151338313No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4939092.0286203168845165No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4549661.8686706885108004No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4455891.8301567664898895No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4309981.7702275102024723No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4193631.7224393601850574No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3868531.5889118348678115No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3850901.5816707082257229No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3832041.573924386701628No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2954731.2135890030163832No Hit
TGTCTTTCCTGCCAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1754030.7204284381181449No Hit
GGTTGCCAGATGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1619230.6650623648706373No Hit
GGTTGCCAGATGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1475420.6059956364305477No Hit
TGTCTTTCCTGCCAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1444840.5934355880632718No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT915330.3759512449973385No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT890980.3659500292438013No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT862360.35419500686736455No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT847850.3482353501698769No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT796110.3269843069219092No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT743880.3055320071762317No Hit
TGATGGCCTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT724660.297637823735452No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT703670.2890166525376391No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT627190.25760420979305904No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT621220.25515216634137045No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC550640.2261630161202347No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT534850.21967762816342357No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT528780.2171845119571003No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG527640.2167162825542653No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT521820.21432584823452872No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC517180.21242007241948097No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC498010.2045464253560186No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT494980.20330192089058874No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG483080.19841426308906543No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT471130.19350606891022482No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC465150.19104991818307276No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT459770.1888402039837286No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT455360.18702889550434057No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT447430.18377182606181286No Hit
GGTTGCCAGATGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT445360.18292162004087562No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG437620.17974258883215372No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC432680.17771359475320203No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG411740.16911295993270642No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT407140.1672236132195125No Hit
TGTCTTTCCTGCCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT400910.16466478060577383No Hit
AAGTATCGTTTCGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT398590.16371189269824998No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC395930.1626193574249683No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG393990.16182254598505608No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC393500.1616212894873463No Hit
AAGTATCGTTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390710.16047535963303958No Hit
TGATGGCCTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT389660.16004409570937575No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG388760.15967444091766392No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC380980.15647898060708818No Hit
TGATGGCCTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC379550.15589164021581267No Hit
TGATGGCCTATTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG372890.153156194757145No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT367250.15083969139575076No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG361080.1483055024347929No Hit
AAGTATCGTTTCGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT357230.1467242013813589No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC353330.14512236395060754No Hit
AGTAAGTTCAGCGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT341200.14014023881342455No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT335780.13791409551222655No Hit
GTCAACTCTTTAGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG331540.1361726107157174No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA324140.13313322687275334No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC320690.13171621683785792No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG313160.12862343841386878No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG295750.1214726718319763No Hit
AGTAAGTTCAGCGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG295210.12125087895694918No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC284020.11665483771333188No Hit
TTCCGCCTCTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT281480.11561158974561178No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC276210.11344705557636575No Hit
GTCAACTCTTTAGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA266790.10957800208978175No Hit
AAGTATCGTTTCGCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA265740.10914673816611793No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC262090.10764758262195322No Hit
GTCAACTCTTTAGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT261390.10736007333951067No Hit
TGATGGCCTATTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC258740.10627164534169246No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT256540.10536804473973017No Hit
AGTAAGTTCAGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG254900.10469445156372191No Hit
TTCCGCCTCTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT253240.10401264383678674No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC250960.10307618503111672No Hit
TTCCGCCTCTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG248460.10204936616525048No Hit
TGATGGCCTATTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA244530.10043520690810874No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACT3924700.082.998011
TGTCTTT2045800.082.932261
TTCCGCC2848750.082.931181
GTCTTTC2044400.082.908062
TCAACTC3932650.082.90372
TCCGCCT2848850.082.847222
TGATGGC4702400.082.8355261
CAACTCT3936100.082.814193
AAGTATC4537400.082.805641
CTTTCCT2044600.082.793934
TCTTTCC2047000.082.780643
CCGCCTC2850150.082.766943
GTATCGT4534050.082.730113
AGTATCG4536850.082.723082
CGCCTCT2848650.082.714874
AACTCTT3939100.082.708174
GCCTCTC2846600.082.682595
ACTCTTT3936750.082.655295
TATCGTT4538850.082.628644
CCTCTCT2835150.082.618986
Produced by FastQC (version 0.11.5)