FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004824545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004824545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37165947
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAGC264150.019.4366467
GTAGAAG259900.019.1748376
AAGCGCT263550.018.91762410
GAAGCGC266150.018.7204239
TTAGTGC245550.018.4992852
GCATAGG243350.018.449997
ACAGTAG321800.018.2440763
CATAGGT259650.018.189598
AGTAGAA279550.018.18895
ACACAGT342950.017.9946581
GTGCATA250100.017.8245325
ATAGGTA252050.017.765169
AGTGCAT258250.017.5047234
AGCGCTA95250.017.4671511
AGAAGCG315300.017.420468
GTTAGTG297500.017.1868361
GGGACCT64000.016.9310841
TGCATAG273000.016.7724386
CAGTAGA310400.016.767294
TAGGTAA267750.016.68650610
Produced by FastQC (version 0.11.5)