FastQCFastQC Report
Mon 13 May 2024
EGAF00004827434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301727953
Total Bases29.7 Gbp
Sequences flagged as poor quality0
Sequence length35-131
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22264340.7378945098931553No Hit
GTTGTCAAGATGCTACCGTTCAGAGCGGCTCGTGCTGCGTCGTCTCAAGT16331000.5412491563219534No Hit
GTTGTCAAGATGCTACCGTTCAGAGTGGATGGGATAAGTGCGTGATGGAC9343270.30965874746116084No Hit
GTTGTCAAGATGCTACCGTTCAGAGTTACCCGCAGGAAGACGTATACCCC6165210.20433009068934357No Hit
GTTGTCAAGATGCTACCGTTCAGAGGCAGCCGGCGTCGTACGAGGCACAG5502720.18237355688420423No Hit
GTTGTCAAGATGCTACCGTTCAGAGATTCAAGGGCAGCCGCGTCACGATT5086150.16856741145226276No Hit
GTTGTCAAGATGCTACCGTTCAGAGATTGGGAGGCTTTCGTACCGCTGCC5065870.16789528280795385No Hit
GTTGTCAAGATGCTACCGTTCAGAGGTGATCCGCGCAGGCACACATACCG4882770.16182690239508568No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4383340.14527457454364529No Hit
GTTGTCAAGATGCTACCGTTCAGAGCCCCACCAGGTTGCTTTGTCGGACG3521640.11671573564813202No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)