FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13868453
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT2301871.6597885863693664No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1695351.222450694392518No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC445600.32130476268694136No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT363840.26235081879716504No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA294570.21240292626726284No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT281530.20300029138073297No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT274080.19762838724694093No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA206100.14861066335228595No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN205190.14795449788091No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT195350.14085925805855923No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA193650.13963345443071407No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA193420.1394676104104762No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT176130.12700046645433344No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG176040.12693557096815342No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT161880.11672534780916084No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG159340.11489385297696866No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC150400.10844756801641826No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC144640.10429425690089587No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC143740.10364530203909549No Hit
CCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTT142450.10271513340384829No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA141950.1023546029250703No Hit
CCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGG139140.10032842163433801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG363750.065.737463
CCAGTAG261550.064.039155
ACCCGGC372950.063.96375
CACCCGG386900.061.8038644
GTAGCAT282300.059.414228
TAGCATA283800.059.1163189
CGGCCCG423150.056.540998
CTCACCC424250.056.4528432
CAGTAGC297600.056.3531346
AGTAGCA298600.056.217517
GCCAGTA311600.053.798844
GCGATTT419200.053.59594394
TGCCAGT317450.052.881643
CCTCACC486950.049.510471
CCTGCCA352450.047.9764141
CTGCCAG351200.047.787422
CGACCGT16600.047.562571
GGCCCGG586100.040.9171529
CCGGCCC592500.040.7367747
CAGTTAT398250.040.1044894
Produced by FastQC (version 0.11.5)