FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13816684
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT2283421.6526541390104892No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1685391.2198223539019928No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC441590.31960635417296945No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT361730.2618066679385589No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA297550.21535558025355434No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT276800.2003375050048188No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT274450.19863666274773312No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN213200.15430619966411624No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA201480.14582370125856536No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT198560.14371031428380357No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA190310.13773927231743882No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA190160.13763070791805038No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT174760.12648476291416957No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG174220.12609393107637115No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT160830.11640274902429557No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG155530.11256680691257034No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC151370.10955595423619734No Hit
CCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTT144160.10433762543892587No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC143870.10412773426677488No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC142670.10325921907166727No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA140020.10134124801580466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG364450.066.5593263
ACCCGGC374000.064.931865
CCAGTAG261750.063.006835
CACCCGG386500.062.7251474
GTAGCAT281150.058.8004958
TAGCATA284850.057.9878589
CTCACCC426500.056.9479522
CGGCCCG427100.056.8942268
AGTAGCA295000.056.1499447
CAGTAGC296150.055.802946
GCGATTT418150.054.51385594
GCCAGTA313850.052.6246834
TGCCAGT317450.052.1911473
CCTCACC481350.050.8005071
CGACCGT16650.049.1239971
CCTGCCA348850.047.781381
CTGCCAG350850.047.2544442
CCGGCCC595150.041.365117
GGCCCGG589350.041.3192189
ACCGTTG20300.040.9817243
Produced by FastQC (version 0.11.5)