FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13859024
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG653370.4714401244993876No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA402800.29064095711213145No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG365200.2635106195068282No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA279080.2013706015661709No Hit
CGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAG236350.1705387046014207No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC230340.16620217989376454No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT219930.15869082844506222No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT200970.14501021139728165No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA198710.14337950493483523No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC194310.1402046781937891No Hit
GTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACT187970.13563004148055446No Hit
CCCAAATCTTTAAACCGATGAGCAACCTCACTCTTGTGTGCATCTCGACG185600.1339199643495819No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT182070.13137288744142445No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC179420.1294607758814762No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC176390.12727447473934672No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA167190.1206362006444321No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG160410.11574408125709286No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA159040.11475555565817622No Hit
CTTCAAATGGACACTGCTGAAGATACTGAGCAAAGGCAATCAACACCAAG155720.11236000457175051No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA153340.11064271192545738No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA152510.11004382415385094No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG151660.10943050535160341No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG143530.10356429139598863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCG133650.049.902951
CCGTCGG186650.035.5305442
CCCGTAT28200.034.8836981
TGTTACG23550.034.5269363
TGTCGAG12500.032.7123765
GTATCGA26400.032.4018864
ACGACTT57350.032.3361361
GTTACGA25250.032.0162054
TTACGAC25650.031.333695
CGGCATG245750.026.8513076
TGTCCGT28400.026.80939394
TTCGCAG249450.026.05727494
TATCGAA35250.025.8669625
TCAATCC90050.025.73081294
TACGACT32450.024.9118296
CATGTAT271100.024.7042089
CGTCGGC273300.024.4547253
GCATGTA277900.023.8460338
GGCATGT278600.023.8367277
GTCGAGG17450.023.1630026
Produced by FastQC (version 0.11.5)