FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13859024
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT2305491.663529841639642No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1704501.2298845863893446No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC446930.32248302622175995No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT361600.2609130339914268No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA295550.21325455529913215No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT281000.2027559805077183No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT280510.20240242025701088No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN226430.1633809134034258No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA205830.1485169518430735No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA196390.14170550538046547No Hit
CTTCTGTCAACCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTAT194600.14041392813808534No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA192600.13897082507397346No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT178790.12900619841628097No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG176070.12704357824908882No Hit
CACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTT162620.11733871014293648No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG159620.11517405554676867No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC149100.10758333342954021No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAAC148820.10738129900056453No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC147040.10609693727350498No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA143350.10343441212021856No Hit
CCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTT141440.10205624869399173No Hit
CCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCAACCCGG140340.10126254200873021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG359950.066.220863
ACCCGGC370650.064.3043065
CCAGTAG264950.064.200015
CACCCGG380550.062.61084
GTAGCAT285150.059.9088868
TAGCATA287750.059.2859089
CAGTAGC298050.057.2211386
CTCACCC418100.057.1025622
AGTAGCA301600.056.641317
CGGCCCG429600.055.5834058
GCCAGTA319350.053.311424
GCGATTT423750.053.2184994
TGCCAGT323800.052.7535253
CCTCACC474450.050.706941
CTGCCAG355700.047.9050942
CCTGCCA358750.047.8515971
CGACCGT17100.043.707761
GGCCCGG587150.040.788779
CCGGCCC597350.040.517127
CAGTTAT408800.040.16055794
Produced by FastQC (version 0.11.5)