FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12287710
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT25600.027.5832041
GTATCGA23550.025.745154
CGTATCG28000.024.1713583
CCGTATC33650.020.2525422
TATCGAA33200.018.6866935
CGGTTCG8050.015.763933
GTCCGAT9000.015.168721
ATCGAAG48250.015.0010546
TATCGCG3250.014.46120486-87
CCGTTAA20050.014.41612430-31
TTGCACG33850.013.8846898
GTCCGTT11850.013.1095891
ACGGTTA22650.012.65764612-13
GGTACCG23450.012.22572920-21
TCTACGG25950.012.134797
ATCGCGC27000.012.09786866-67
TCGAAGG63950.011.9060957
ATCGCGT3207.706988E-411.74972694
CGCGTAA8701.8189894E-1211.3630141
CTCCGAT50250.011.3355551
Produced by FastQC (version 0.11.5)