FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12287710
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN174780.142239685018608No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC136560.11113543532521519No Hit
CGCGGGTCTGTCTCTTGCTTCAACAGTGTTTGGACGGAACAGATCCGGGG130010.10580490587749874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT14200.030.7774221
CATTCGG31200.026.5094684
CGCGGGT56350.026.436441
GCATTCG31000.026.3769863
GCGGGTC60850.024.9459932
GCGATTT28550.023.21294894
CCGTTGA20900.020.6863784
CCGTCAT70900.020.6797831
ACCGTTG20550.020.5810873
GACCGTT20850.020.5113472
TACCGTA54450.020.455139
CGGGTCT73500.020.395813
AACCCGT35850.020.1878662
CTACCGT56000.019.8890428
ACTACCG55600.019.8628397
CGACCTA12050.019.4996094
ATTCGGG43250.019.1233585
GTAGGTC27700.018.8322452
CAACCCG39700.018.5843241
TACTCTA80400.018.5291299
Produced by FastQC (version 0.11.5)