FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14295048
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA380800.2663859540730468No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC277460.1940951859692951No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN265880.1859944786474309No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG225070.15744613099585256No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC216800.15166091082730188No Hit
CCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAGC189910.1328502009926794No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGG179160.12533011431651017No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT145220.10158762670821392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT23050.039.56331
GTTGACT113200.036.41791
ACCGTTG29350.030.9101983
GCGATTT37050.030.82849994
GACTATT132150.030.233554
GACCGTT30600.029.6481512
TTGACTA149400.026.5554452
TGACTAT151100.026.3183383
GTAGGTC41000.026.2550812
CCGTTGA35600.025.878764
ACGTGCG29850.025.6621078
ACATGCC123200.025.5638242
GTCTAAC32750.025.261731
CCGTCAT80200.024.6170851
ACCCGGC49050.024.051135
TCACCCG55300.023.0354523
TGCCTAT136600.022.605575
AACCCGT47400.022.4125792
GCCTATC140950.021.7717116
CTCGATT31100.021.76907548-49
Produced by FastQC (version 0.11.5)