FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13409354
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1050180.7831697186904009No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA815030.6078070576703396No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT486780.3630152503990871No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC458020.3415675356173012No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC405610.3024828787427045No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC401860.299686323442576No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC385690.2876275769884217No Hit
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCC362760.2705275735132356No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC359380.26800694500271977No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA354430.26431549200655No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC346630.25849865698228264No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC311170.23205443006426707No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA308170.22981718582416427No Hit
CGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCC293770.2190784134716706No Hit
CACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCG292030.21778081181241093No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG279690.2085782805047879No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC277200.20672136778550257No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC262840.1960124253562103No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT261240.19481922842815547No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA250540.18683972397178866No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT244810.18256658747319224No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT243120.18130627321793427No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC235460.1755938429248717No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT219160.1634381492203129No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT214450.15992567576335145No Hit
CCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTC213410.1591500977601158No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC199430.14872453960123658No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA192830.1438026022730103No Hit
GTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGC190640.14216941397773525No Hit
GGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCC188800.14079723751047218No Hit
CGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCC175930.13119945972043098No Hit
CTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCA174360.13002863523477715No Hit
GTGATGTTATAAGCCTAAGGCAGCTTGACTTGCAGCAACAAGTTTTTTAC173460.1293574619627463No Hit
CTTCATTTTCTTTCTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATG173130.12911136509633497No Hit
GGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCC162920.12149727719918499No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT156070.11638890285095016No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC154920.11553129255891074No Hit
GTCAAAAGAAAGCTCTTTATTGATTGACTCAGCAATGCAGGGCTGGCACC148750.11093002690509923No Hit
CTCCTGTCCACTCCCGTCCACTTTGCCCTCTTCCAGGCTGAAATCTTGCT148580.11080324973149341No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA146180.10901345433941113No Hit
GTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGC145240.10831245114417891No Hit
CCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAG142850.10653011323289696No Hit
CCACATCTTATCGCATCCACTCCTGTCCACTCCCGTCCACTTTGCCCTCT142310.10612740926967845No Hit
CCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGC140900.10507590447683013No Hit
GTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCT138340.10316678939194238No Hit
CACGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCC137130.1022644342151009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAA9600.045.1064381
GGTTCGG21700.043.7518234
GTCGGTA8050.039.7589531
CGGTTCG27500.039.6515163
GTCGGTT28100.037.185071
GTCGGGA92600.037.054291
TCGGTCC26950.034.7047657
GAACGGG98050.034.3210229
AGGAACG100200.033.4438747
CGGTAAT12700.033.3075942
GACGGTT11100.033.0745241
TCGGTAT11200.028.9558012
TCGGGAG116450.028.858322
GGAACGG120400.027.754798
GTCGTAT2451.2732926E-1124.9746381
CGTCGAC1901.3353201E-824.737223
GAGGAAC146150.023.4760656
CCCGATT20250.023.44154794
ACGCATA9750.023.1380482-83
TCGCACT24000.022.5206749
Produced by FastQC (version 0.11.5)