FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13322944
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1030430.7734251528791234No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA800810.6010758583087942No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT479400.3598303798319651No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC451360.3387839804775882No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC398360.2990029831244506No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC397710.29851510296823286No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC378510.284103873738417No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC356820.26782368821785935No Hit
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCC356600.26765855954960105No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA348070.26125607073031304No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC333670.2504476488079512No Hit
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA308680.23169053326351896No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC303300.22765238673974764No Hit
CGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCC290730.21821753510335254No Hit
CACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCG283190.21255812529122695No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC276690.20767932372904968No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG273400.20520989955373228No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC253580.19033330771337026No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT251800.18899726667018943No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT245320.18413347680512657No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA245050.18393081889408225No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT239130.17948735654822237No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC230230.17280715133231814No Hit
AACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTT213320.1601147614221001No Hit
CCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTC211300.15859858001354654No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT211190.1585160156794174No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC198820.14923128101416622No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA194120.14570353219228424No Hit
GGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCC184200.13825773042354603No Hit
GTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGC184100.13818267193797407No Hit
CTTCATTTTCTTTCTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATG176330.13235062760903296No Hit
CTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCA174180.13073687016923588No Hit
GTGATGTTATAAGCCTAAGGCAGCTTGACTTGCAGCAACAAGTTTTTTAC172910.129783627402472No Hit
CGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCC171010.12835751617660482No Hit
GGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCC160460.12043884594876329No Hit
CTCTTTTGTTGCCTTGGGCTTGTGTTTCACAAGCTCAACAAGTGCAGTTT156010.11709874334081116No Hit
CCCTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTC154610.11604792454280376No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA148690.11160446219694387No Hit
GTCAAAAGAAAGCTCTTTATTGATTGACTCAGCAATGCAGGGCTGGCACC148490.11145434522579994No Hit
CTCCTGTCCACTCCCGTCCACTTTGCCCTCTTCCAGGCTGAAATCTTGCT143210.1074912571876006No Hit
CCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAG140950.10579493541367435No Hit
GTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGC140070.10513442074064111No Hit
CCACATCTTATCGCATCCACTCCTGTCCACTCCCGTCCACTTTGCCCTCT139790.10492425698103963No Hit
CCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGC138120.10367078027198792No Hit
CACGTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCC135870.1019819643466189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAA9950.049.2017021
GGTTCGG20650.041.653674
GTCGGTA7850.041.3760761
GTCGGGA93750.038.2607571
GAACGGG100800.035.0160759
AGGAACG101700.034.659987
CGGTTCG28750.034.3323673
TCGGTCC26350.032.8189937
GTCGGTT28150.032.7753641
CGGTAAT15050.032.4802322
TCGGTAT10500.030.887452
TCGGGAG117950.030.405272
GGAACGG119800.029.4626058
GACGGTT12450.025.332341
GGTTCGC19800.024.9256445
GAGGAAC144600.024.6703076
ACGCATA10900.023.9306582-83
CGGGAGG150900.023.4857463
TCGCACT25850.023.4540149
GTTCGCC21000.023.2763636
Produced by FastQC (version 0.11.5)