FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12711851
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN187610.14758668898809466No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC175810.13830401253129856No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA168350.1324354730086122No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC128730.10126770680367476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT16150.037.2471051
GTCTAAC36650.033.0826871
ACGTGCG36350.032.8392268
GCGATTT37700.032.41545594
ACCCGGC45200.029.3194265
TCACCCG47700.028.9648973
ACCGTTG21000.028.8677863
AACACGT42900.028.8099985
CATTCGG24050.028.5287384
ACTACCG48500.027.7125137
CTACCGT50150.027.1763238
CACCCGG49750.026.732414
GACCGTT23300.026.4232272
GTAGGTC35050.025.7444532
TAACACG48300.025.199824
CGTGCGC48200.025.1555949
CGCAAAA42600.024.711181
CTCGATT32900.023.79589348-49
ACACGTG53250.023.1200526
TCAGACG48850.023.0896382
Produced by FastQC (version 0.11.5)