FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12695082
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN188920.14881353267351877No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC175740.13843155955983585No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA164300.12942019594674536No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC127620.10052711750896921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT16750.044.6215971
ACCGTTG22250.034.011123
GTCTAAC35000.032.36771
GACCGTT24450.030.376732
ACGTGCG36900.030.0553368
GCGATTT36600.029.79304194
ACTACCG48900.029.5027227
CCGTTGA26300.028.5947234
AACACGT40700.027.8311145
ACCCGGC43800.027.6855745
GTAGGTC32550.027.149992
CTACCGT54500.026.2127768
TCACCCG49450.025.1886393
CATTCGG22150.025.0397474
CACCCGG49600.024.6384474
CGTGCGC45700.024.5767569
TAACACG47050.023.6761134
GTTGACT86700.023.367971
CTCGATT31850.022.88199848-49
CGCAAAA47600.022.1209551
Produced by FastQC (version 0.11.5)