FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12704118
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA199350.15691762308882837No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA133540.10511552238415922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT32550.034.1226431
GTATCGA30200.033.150224
TATCGAA38800.026.529325
CGTATCG56150.022.9358353
CTTAACG63750.022.2659153
CCGTATC59350.021.6199992
CGCGTAT2005.8961086E-721.178451
AACGGTT68700.020.8667666
TTAACGG69500.020.356144
ATCGAAG65500.019.0876466
TAACGGT77450.018.9341985
AGTTTAC140300.018.15639994
GTCCGAT6050.017.11391
CCCGTCG17600.016.8464931
CCGTTAA27000.016.53653530-31
TCTTAAC92700.016.1235682
CGGTTCG5250.016.1148763
TCGAAGG77200.015.7676577
GGTACCG29350.015.13289620-21
CTTCGCG14350.015.0666936
Produced by FastQC (version 0.11.5)