FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13908759
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA142200.1022377337906279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT24100.029.6908681
GTATCGA21400.028.3323884
CCCGTCG24500.019.4066981
CGTATCG48650.018.066173
CCGTATC53850.017.4562742
CGAAAGG54000.017.2329648
CTTAACG39650.016.8326473
TTCGAAA55900.016.8157446
TATCGAA40050.016.7818875
GCGTTTA14750.015.9325494
CCCGGTT19800.015.9296411
CTCGTTT73550.015.48920251
AGTTTAC114800.015.47527894
ATCGCGT8600.015.30199794
ATCGAAG62650.014.6288936
TCGACCG8350.014.35298530-31
TGTCGAG12950.014.1547485
GTCCGAT9800.013.9305521
GTCCGTT11850.013.9042021
AATATCG27600.013.793486
Produced by FastQC (version 0.11.5)