FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13908759
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN199990.1437870912854267No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT175440.12613634329274093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT15050.036.8474851
GCGATTT51450.033.3441794
ACCCGGC57150.032.235455
CATTCGG37100.031.7954734
TCACCCG59300.031.2253283
ACCGTTG19550.029.3277343
CACCCGG67250.027.7436564
GCATTCG41550.027.7115293
GTCTAAC30400.025.971561
CTCGATT41600.024.9237848-49
ACGTGCG31850.024.346348
GACCGTT22900.024.0120012
CTAGGAC43650.023.5788291
CGGCCCG81350.022.9924228
TAACACG35250.022.3983294
ATTCCGT47150.022.13856952-53
TCAGACG40400.021.9866182
CCGTTGA26000.021.6906914
AACACGT37000.021.4658895
CCAGTAG55350.020.9721625
Produced by FastQC (version 0.11.5)