FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13901575
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA145400.10459246524224773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT21450.029.4017721
GTATCGA20350.028.4095654
CGTATCG46850.019.1623023
CCGTATC50650.018.281452
CTTAACG39050.018.054863
TATCGAA36550.018.0038155
CCCGTCG24550.017.253891
AGTTTAC119450.017.11555794
GTCCGTT10500.015.6882591
AACGGTT47250.015.1198296
CGAAAGG55450.014.8327928
TTCGAAA55750.014.7536116
TCGACCG7850.014.66842830-31
GCGTTTA14550.014.5369544
CGCGTAT2308.655985E-414.3052053
GTCGTCT53350.014.291461
ATCGCGT6650.014.13501694
TCGTCTC43350.013.8785712
ATCGAAG60350.013.6290616
CTCGTTT79200.013.5489511
Produced by FastQC (version 0.11.5)