FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13279843
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT24350.032.093061
GTATCGA23200.030.9954834
TATCGAA35300.021.9686955
CGTATCG48500.021.0287283
CGCGTAT2109.3960625E-720.175541
CCGTATC52650.018.9248522
GCGTTTA16250.016.7752744
CGTTTAA17450.016.1603535
CGAAAGG49100.016.081428
ATCGAAG58800.015.9064196
GTCCGAT8650.015.2385591
ACGTACG4500.015.14411752-53
ACGTTCG6950.014.8776566
TTCGAAA54250.014.6414336
AGTTTAC102900.014.61581694
CCCGGTT15650.014.4387261
GTCGTCT57250.014.3901231
TCTACGG26600.014.311997
CTTAACG36550.014.2735043
CTCGTTT72050.014.2437541
Produced by FastQC (version 0.11.5)