FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13279843
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN210360.1584054871733047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCGG32100.035.8676034
CGACCGT13650.034.772031
GCATTCG35050.032.8486673
GCGATTT38200.028.67008294
ACCGTTG17950.026.4421393
ACCCGGC45850.026.1360845
ATTCGGG46700.025.6603765
ACGTGCG25300.025.2617268
CTACCGT36800.024.5187348
GTCTAAC26550.024.2491971
ACTACCG37600.024.1217677
TCACCCG50900.023.9123173
CACCCGG53750.022.0324824
ACGTGCC56900.021.969852
CTCGATT32150.021.93901848-49
AACACGT30400.021.7964215
TCAGACG38100.021.7092782
GACCGTT21600.021.5396772
CCGTTGA22100.021.26424
TCGGGCC58150.021.1734317
Produced by FastQC (version 0.11.5)