FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13250664
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN218150.16463325913327814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT12850.035.8498341
CATTCGG35100.034.8171044
GCATTCG37400.032.9276733
GCGATTT39850.027.95289294
GTCTAAC22900.026.4800261
ACCCGGC45050.025.3531175
TCACCCG48300.025.2048443
ACGTGCG23100.025.0223248
ATTCGGG50200.024.8120165
ACCGTTG19950.024.0319293
CTTAGCG12550.022.841298
GACCGTT21500.022.519722
CACCCGG52650.021.961564
CCGTTGA21150.021.7792134
AACACGT27550.021.667165
CTCGATT31500.021.56891348-49
ACGTGCC53900.021.36692
TAACACG27200.021.2550754
CTACCGT37950.021.0509478
ACTACCG37400.020.9831737
Produced by FastQC (version 0.11.5)