FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13254213
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT25700.033.3297581
GTATCGA24400.031.0132964
TATCGAA38050.021.4934675
CGTATCG51750.020.0721443
CCGTATC55350.019.1063082
GCGTTTA17200.016.6691444
CGTTTAA18950.016.617955
ACGTTCG5400.016.5371976
GTCCGAT8550.016.5138761
ATCGAAG62700.016.3414656
CTTAACG40650.016.303113
ACGTACG4950.016.1409652-53
TCTACGG26600.015.9018087
CGTACGA5400.015.66625654-55
CGAAAGG47100.014.1694548
CGCTAGT2350.001008424214.0192271
TGTCGAG11950.013.7661345
AGTTTAC101550.013.65314394
CCCGGTT16650.013.5681591
TCCGACG10050.013.5626272
Produced by FastQC (version 0.11.5)