FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13869238
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG205190.14794612364428383No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT138730.1000271247778717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT23200.029.0132031
CCCGTCG45800.028.2627771
ACGACTT29400.027.5377661
CGCGTAT1955.966285E-1026.5525171
GTATCGA21600.026.5462674
CGTATCG39100.022.9590533
TTACGAC11900.022.5125795
CCGTCGG59650.021.1952822
TGTTACG12850.020.8482253
CGGCATG91300.020.7458693
GTTACGA13200.020.6514914
CCGTATC42750.020.559042
GTTCGAC33200.019.04033328-29
TCGACCG33250.018.7996430-31
TATCGAA34900.018.7191755
GTCCGTT11200.018.071661
ACGTTCG25500.017.8784756
AAGTTCG37550.017.08495526-27
CGTCGGC75200.016.8124793
TGTATTA113500.016.5224488
Produced by FastQC (version 0.11.5)