FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13834795
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG204550.14785184746141883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT24300.031.9583231
GTATCGA22850.029.209084
CCCGTCG43000.028.8962691
ACGACTT30500.026.5420761
CGTATCG39450.024.305183
CGGCATG90000.023.6054133
TTACGAC11700.022.0949235
CCGTATC45900.021.401752
ACGTTCG25800.021.1319626
TATCGAA34950.021.1138865
GTTACGA12050.020.2829914
CCGTCGG60600.020.1658442
TGTTACG12500.019.5528033
TCGACCG32300.019.20703930-31
TGTATTA113500.018.7996168
TGTCCGT27050.018.76493694
GTTCGAC33350.018.46139328-29
CGTTCGA30200.017.8969887
TACGAGC20050.017.58126
TCGGCAT136800.017.5570092
Produced by FastQC (version 0.11.5)