FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13851094
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT895580.6465770862575909No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG552360.3987843848290973No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN203800.1471363922589797No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC185720.1340832716895864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG159200.057.1335263
ACCCGGC161300.056.3558585
CACCCGG171900.052.9388774
CGGCCCG179350.050.7299468
GCGATTT181100.047.36559394
CCAGTAG116550.047.3448525
GCCAGTA122850.045.302464
GTAGCAT134700.041.8323258
TAGCATA136050.041.1408849
AGTAGCA136750.040.72387
CAGTAGC137650.040.389256
GGCCCGG238700.038.4118549
CTCACCC244150.037.466432
CCGGCCC253300.036.5315257
TGCCAGT155300.035.8976753
CCCGGCC266400.034.541076
CGACCGT15650.034.2410241
CATTCGG33650.033.804584
CGGACAC165150.033.32174712-13
CTCGATT167800.032.3741748-49
Produced by FastQC (version 0.11.5)