FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14979185
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTA430750.2875657120197127No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTT317830.2121811033110279No Hit
CTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACAA266870.1781605608048769No Hit
CTCTTATTCTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGC238290.1590807510555481No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA204340.1364159665562579No Hit
CCTCGGCAAAGCAGGTCTCCTTATCGTCAGCCTTGCAGCACTTCTCTACA202190.13498064147014674No Hit
CTCATGGTAGGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCA179780.12001988092142529No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG159510.10648776952818194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT46100.036.351471
GTATCGA45450.035.9874344
TATCGAA51500.031.942315
CGTATCG78250.027.5698173
CCGTATC79850.027.0762462
CGGTTCG7500.023.1870773
ATCGAAG82700.023.130776
CCGGTAA12050.021.4856551
CCCGTCG19700.021.0275841
TCGAAGG94850.019.9196287
TGTCGAG11550.018.7189465
CGGTAAT12200.018.492132
GTCGAGG13100.017.2215046
CCGTTAA29850.017.16209630-31
TATCGCG3200.016.89024786-87
CGAAGGC98100.016.8163078
GACGTGT21000.016.56179
ACGGTTA32000.016.52320512-13
ACGTTCG6900.016.3479526
GGTACCG32650.016.05017720-21
Produced by FastQC (version 0.11.5)