FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004827484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004827484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14979185
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACAAGCCC382490.25534767078449194No Hit
GACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACAAGCCC356000.23766313053747581No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTA270700.1807174422373447No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCC259630.1733271870265305No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN242770.16207156797916578No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT207700.13865907924897114No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTTGTGAAACACA205310.1370635318276662No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA198200.13231694514754974No Hit
GGAGAGACAAATCAAGAAACAAACTGCACTTGTTGAGCTCGTGAAACACA188860.12608162593625755No Hit
CTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGG180590.12056063130270439No Hit
CCTAGAGGCAGCTGCTCCAGGAACAGAGGTGCCATGCAGCCCCGGGTACT178380.11908525063279478No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC169640.11325048725948708No Hit
CCCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTT167150.11158818053185136No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT165140.11024631847460327No Hit
CCCACACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTT160340.10704187177072717No Hit
CTCATTCTTGGCAGGATGGCTTCTCATCGTCTGCTCCTCCTCTGCCTTGC159860.10672142710033956No Hit
CAAGAAAGTACCCCAAGTGTCAACTCCAACTCTTGTAGAGGTCTCAAGAA158850.10604715810639898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT21950.054.3826371
ACCGTTG27450.043.827393
GCGATTT48200.042.4186794
GACCGTT30650.039.4077072
ACCCGGC56700.039.286365
CACCCGG66200.033.9328844
TCACCCG69850.032.8323063
GTCTAAC29100.032.6226651
CCGTTGA37350.032.0848854
CGGCCCG75550.029.2985448
CTCGATT44750.029.0010748-49
GTTGACT103950.028.7536951
CGCAAAA56300.028.7151551
ACGTGCG33350.028.042518
CGGACAC48750.027.57122812-13
ATTCCGT48200.026.53362552-53
ACTACCG67150.025.9644327
CGTTCTT51100.025.29135776-77
TAACCCC62950.025.2335959
CCAGTAG44550.025.0004145
Produced by FastQC (version 0.11.5)